These samples are handled according to the strict guidelines of the Israeli National Database for Biological Samples (MIDGAM).

The biobank team includes a project manager, laboratory manager, and seven laboratory technicians and recruiters. The clinical staff who treat the patients also actively participate in the collection process.

 After obtaining signed informed consent from patients, medical personnel collect tissue or blood during treatment or surgery and transfer it to the biobank for processing and storage. This collaboration between the clinical team and the biobank's operations team is essential for the smooth operation of the NBB on a daily basis.

NBB has strong academic partnerships with researchers from Ben-Gurion University of the Negev, the Weizmann Institute, Ariel University, and Hebrew University in Jerusalem. Additionally, the biobank has collaborated with the biotech industry, providing blood and urine samples to companies working on medical devices and tools for early diagnosis and prediction of various health conditions over the past two years. The NBB has ongoing collaborations with Memed, Imunai, Giluii, ProticaBio and Starferpharma.

As of 2023, the biobank has collected approximately 40,000 biological samples from around 6,000 patients.